Asda workers at the Brighton Hollingbury superstore have announced strike dates over the Bank Holiday weekend.
It follows industrial action at the Gosport, Wisbech, and Lowestoft sites.
GMB Union members at the Brighton store will walk out from 8pm on 24 May until 3pm on Saturday, 25 May.
Over 100 staff members plan to strike over issues such as hour cuts, inadequate training, high-pressure work rates, and unresolved health and safety concerns.
The union also cited payroll problems, incorrect pay, delays in resolving equal pay disputes, and the lack of collective bargaining with GMB as additional reasons for the strike.
GMB regional organiser Declan MacIntyre said: “Asda workers at the Brighton Hollingbury superstore are following colleagues in Gosport, Wisbech and Lowestoft in taking strike action.
“There are further stores across the South East where members have voted overwhelmingly to be balloted formally and where formal industrial action ballots may well follow next.”
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He continued: “Asda management would do well to listen to their own staff and seek to address this dossier of issues before further disruption is caused to their loyal customers.”
“Our members are clear that Asda need to provide more staff hours to the Hollingbury superstore to help prevent existing staff being overworked and put at unnecessary risk of a health and safety catastrophe.”